Recombinant Human BMP-2Product SpecificationsExpression of Human Proteins in Human CellsExtreme low EndotoxinHigh PurityAnimal Free and Xeno FreeTag FreeSource: Human cells derivedStructure: Non-glycosylated homodimerPurity: >95% by SDS-PAGEEndotoxin Level: <0.5EU/ugMolecular Weight: 30-38kDaFormulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier proteinActivity AssayThe activity was measured by its ability to induce alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line).ReconstitutionBriefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.Protein DescriptionBone morphogenetic protein 2 (BMP-2) is a member of transforming growth factor β family that includes more than 20 structurally related bone growth factors. BMP-2 is expressed abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. BMP-2 plays an important role in inducing bone and cartilage formation. Mature human BMP-2 is usually a homodimer consisting of two monomers of 114 amino acids. Dimerization is facilitated by a disulfide bridge formed between the monomer, which contains three intrachain disulfide bridges arranged in a cystine knot motif.ReferencesChen D, et al. (2004) Growth Factors 22:233.Wozney JM, et al. (1988) Science 242,1528-1534
